DNA Barcoding and Phylogeny of Acari Species Based on ITS and COI Markers

Acari harbor numerous minute species of agricultural economic importance, mainly Tetranychidae and Phytoseiidae. Great efforts have been established by means recovering morphological, molecular, phylogenetic traits for identification. Traditional identification still relies on external diagnostic characters, which are limited usually exhibit large phenotypic plasticity within the species, rendering them useless delimitation We decided to increase number sequences mitochondrial COI (Cytochrome C oxidase I) marker ITS nuclear ribosomal DNA region in The molecular data allow us establish boundaries relationships among several clades Acari, Sequence comparisons between complete COI, ITS1-5,8S-ITS2, ITS2 all demonstrated that selected regions, even small, gave enough informative positions both species’ studies. Analyses regions unveiled their use as with special emphasis Phytoseiidae comparison Folmer fragment, has universally used a barcode marker. is also suitable dataset Our analyses congruent other recent works, showing monophyletic group, Astigmata, Ixodida, Mesostigmata, Oribatida, Prostigmata monophyletic.